Research was to
study pathogenic bacteria causes of infections. The research question was
whether the bacteria potent or important with the number of species estimation.
The method used a selective differential medium for isolation and determination
of members of species of bacteria. Colorants were used for inhibition of growth
of most positive organisms. The results showed the bacteria gave a positive
result in the stain test and classification in accordance to the cell membrane.
This was a method of differentiation of bacterial species. The negative and
positive organisms were differentiated from each by lack of similarities in
cell membrane. These affected many regions of cell inclusion of usage up and
retainment of stains. It can be concluded stain technique was preliminary
identification of bacteria where application of purple color and decolorization
with a red. The cell walls of certain bacteria retained the initial and some
loss appearance of the final in the membrane.